Journal of Bacteriology & Parasitology

The detection of vector-borne-disease-related DNA in human stool paves the way to large epidemiological studies

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Alpha Kabinet Keita

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

The detection of Plasmodium spp. by the molecular analysis of human feces was reported to be comparable to detection in the
blood. We believe that for epidemiological studies using molecular tools, it would be simpler to use feces, which are easier
to obtain and require no training for their collection. Our aim was to evaluate the usefulness of feces for the detection of these
pathogens towards developing a new tool for their surveillance. Between 2008 and 2010, 451 human fecal samples were collected
in two Senegalese villages in which malaria and rickettsioses are endemic. Rickettsia and Plasmodium DNA were detected using
quantitative PCR (qPCR) targeting Rickettsia of the spotted fever group (SFG), R. felis and Plasmodium spp. Two different sequences
were systematically targeted for each pathogen. Twenty of the 451 fecal samples (4.4%) were positive for Rickettsia spp., including 8
for R. felis. Inhabitants of Dielmo were more affected (18/230, 7.8%; p=0.0008) compared to those of Ndiop (2/221, 0.9%). Children
under 15 years of age were more often positive (19/285, 6.7%) than were older children (1/166, 0.6%; p=0.005, odds ratio (OR)
=11.79). Only one sample was positive for Plasmodium spp. This prevalence is similar to that found in the blood of the Senegalese
population reported previously. This preliminary report provides a proof of concept for the use of feces for detecting human
pathogens, including microorganisms that do not cause gastroenteritis, in epidemiological studies.

Biography :

Alpha Kabinet Keita obtained his medical degree at the Faculty of Medicine of the Gamal Abdel Nasser University in Guinea-Conakry in 2007, completed Master
degree in 2010 and PhD in infectious diseases and Microbiology in 2013 at Aix-Marseille University in France. Currently, he is Post doctoral Research Scientist at
Institut de Recherche pour le Développement (IRD) in Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) at Dakar (Senegal).
He has published 09 scientific papers in reputed journals and his research interests include epidemiology of infectious diseases, Tropheryma whipplei agent of
Whipple’s disease, malaria and non malaria fever.