alexa The Differences Of Apoptosis And Pulmonary Fibrosis Following Sulfur Mustard-induced Acute Pulmonary Injury Via Intraperitoneal Injection And Intratracheal Instillation In Rats | 67800
ISSN: 2161-0495

Journal of Clinical Toxicology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

9th Euro-Global Summit on Toxicology and Applied Pharmacology
June 22-24, 2017 Paris, France

Zhu Xiaoji, Han Wei, Bei Yuanyuan, Zhao Jian and Zhong Yuxu
The 89th Hospital of PLA, China
Jinan Military General Hospital, China
Weifang Medical College, China
Academy of Military Medical Sciences, China
Posters & Accepted Abstracts: J Clin Toxicol
DOI: 10.4172/2161-0495-C1-025
Abstract
Objective: Sulfur mustard (SM) is a vesicant alkylating agent. There are still biochemical mechanisms underlying SM damage that are unknown. This study was to determine the differences of apoptosis and pulmonary fibrosis underlying SM-induced acute pulmonary injury via intraperitoneal injection and intratracheal instillation in rats. Methods: SM-induced changes in apoptosis and pulmonary fibrosis were observed in intraperitoneal and tracheal SM groups that were injected intraperitoneally and instilled intratracheally with 0.1 mL of diluted SM (0.96 LD50=8 mg/kg) and SM (0.98 LD50=2 mg/kg), respectively, and an untreated control group. Results: In the alveolar septum, the positive expression ratio of apoptotic cells, Bax, Bcl-2, caspase-3, and caspase-9 by TUNEL and immunohistochemistry (streptavidin-perosidase method) in the intraperitoneal SM group was increased compared with the tracheal SM group (P<0.05). A significantly lower positive expression ratio of Bcl-2 was detected (P<0.05). Electron microscopic observations confirmed that the type I and II alveolar epithelial cells in lungs exhibited apoptotic morphologic features, such as cracked, lost, and disordered microvilli of membranes, fuzzy mitochondrial cristae, and detached, dissociated ribosomes from the surface of the rough endoplasmic reticulum. A significantly higher positive expression ratio of MMP-2, MMP-9, TIMP-1, TIMP-2, collagen type I, collagen type III, TGF-β1, and Smad7 by immunohistochemical staining in the alveolar septum were detected in the intraperitoneal SM group compared with the tracheal SM group (P<0.05). Conclusions: These data demonstrated increased apoptosis and pulmonary fibrosis via intraperitoneal injection under similar SM LD50 doses in rats.
Biography

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]icsonline.com

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version