By applying enhanced cDNA transfection in mice with hepatocellular carcinoma without metastatic cell lines
(Hepa1-6) COL8A1, and observing cancer cell proliferation, invasion, tumor formation in vivo, this study aims at regulating the
influence of COL8A1 expression on tumor cell drug sensitivity and tumorigenicity.
The COL8A1 expression in transfected Hepa1-6 cells was analyzed with the use of RT-PCR, Western Blot. The
transfection of ? Hepa1-6 cells of invasion ability ? was tested in invasive experiments in vivo and transfection of ?Hepa1-6 cells
of the tumorigenic?ability was tested in vivo tumor. Transfection of Hepa1-6 cells proliferation and its effect on d-limonene
susceptibility was analyzed using the method of MTT.
Expression of COL8A1 in experimental group cell of Hepa1-6 / COL8A1 showed a significant increase when compared
to un-transfected cells of Hepa1-6 control group and empty plasmid transfected cells of Hepa1-6 / Mock control group.
The ability of cell proliferation, matrix adhesion through the invasion and tumorigenic in vivo were enhanced and at the same
time the cells of d-limonene susceptibility were down-regulated .
COL8A1 expression in hepatocarcinoma cells in mice is related to tumor cell proliferation, invasion, in vivo
tumorigenicity and antitumor drug sensitivity, which may provide new targets for tumor chemical therapy.
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