Six species of Bifidobacteria were tested towards enteropathogenic E. coli (EPEC) by the spot on-lawn and the well diffusion
methods. The six strains were active with the both tests; however Bifidobacterium infantis showed the greatest effect with
inhibition zones diameters of 18 and 12 mm respectively. The inhibitory activity of the supernatant was assumed to be due
to bacteriocin-like substances since the treatment with three proteases (pepsin, papain, trypsin) resulted in a decrease in the
inhibition zones diameters. These indicate that the active substances are of proteinaceous in nature. The active substances were
efficiently concentrated using ammonium sulphate (60%) precipitation method followed by deionisation and partial purification
on Sephadex G-75 gel. From the results obtained in vitro, the partially purified bacteriocin-like substance was used in an in vivo
study using young holoxenic wistar mice. Administration of skimmed milk containing 160 AU/ml of the active substance to
diarrheic mice infected with EPEC induced an arrest of the diarrhea concomitant with recovery of a good health status of the
treated mice compared to the untreated ones.
Farida Bendali has completed her Ph.D in 2009 and her ?habilitation [HDR]? "Habilitation ? Diriger des Recherches") in 2011 from Bejaia University.
She is a searcher at the Applied Microbiology Laboratory (Bejaia University). Her research interest is on Molecular microbiology, particularly in
relation with Food Safety, Probiotics and Bacteriocins, Adhesion and biofilm formation of Lactic acid bacteria and pathogens on biotic and abiotic
surfaces. She is a teacher at the Microbiology Department, Nature and Life Sciences Faculty, Bejaia University (Algeria), She is a Professor
associate since 2011.
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