e commonly used prosthesis materials, such as poly(ethylene terephthalate) (PET) known
also as Dacron, have been extensively used in large and middle-sized artery reconstruction.
However, application of these frequently used materials is limited in small-caliber graft
s with an internal diameter smaller than 6 mm are prone to fail mainly due to their
thrombogenicity and poor haemodynamics. One of the possible solutions of these problems
may be reconstruction of the
on the synthetic graft
s. For this purpose,
special PET foils were prepared.
Twelve-μm thick foil was irradiated by krypton ions, ultraviolet light and etched by 1M
sodium hydroxide to obtain pores of defi
ned diameter (from 0,2 μm to 2 μm) and density (4x10
is study investigated the infl
uence of pore size and topography of the PET surface
on the growth and viability of vascular smooth muscle cells (VSMC) and endothelial cells. It
rmed that surface micropores modulate the adhesion, proliferation and viability of
vascular cells. It seems that endothelial cells prefer pores around 1 μm. VSMC were usually
without preference of a specifi
c pore size, only on scaff
olds with 2 μm large pores, decreased
proliferation and viability was observed. According to these results, PET foils with 1 μm large
pores were chosen for further experiments, in which these porous PET membranes could serve
as synthetic analogues of internal elastic lamina separating vascular smooth muscle cells and
endothelial cells in a newly constructed bioartifi
cial vascular wall.
Jana Havlikova has completed her MD studies at The Institute of Chemical Technology (ICT), Prague, Czech
Republic in 2009. She is a second year PhD student in the Department of Growth and Differentiation of Cell
Populations, Institute of Physiology, Academy of Sciences of the Czech Republic.
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