alexa The Pleiotropic Effects Of Fisetin And Hesperetin On Human Acute Promyelocytic And Chronic Myeloid Leukemia Cells
ISSN: 2155-9600

Journal of Nutrition & Food Sciences
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

10th World Congress on Nutrition & Food Sciences
May 29-31, 2017 Osaka, Japan

Aysun Adan and Yusuf Baran
Abdullah Gul University, Turkey
Posters & Accepted Abstracts: J Nutr Food Sci
DOI: 10.4172/2155-9600-C1-045
The studies elucidating the roles and the mechanisms of action of fisetin and hesperetin, plant flavonoids, in acute promyelocytic leukemia (APL) and chronic myeloid leukemia (CML) are absent. In this study, we investigated the mechanism of the antiproliferative and apoptotic actions exerted by fisetin and hesperetin on human HL60 APL and human K562 CML cells. The viability of HL60 and K562 cells was evaluated using the MTT assay, apoptosis by annexin V/propidium iodide (PI) staining and cell cycle distribution using flow cytometry, and changes in caspase-3 enzyme activity and mitochondrial transmembrane potential were determined. Moreover, we performed whole-genome microarray gene expression analysis to reveal genes and biological networks affected by KEGG and IPA analysis. For APL cells, both flavonoids showed a concentration- and time-dependent inhibition of proliferation and induced G2/M arrest and G0/G1 arrest for hesperetin at only the highest concentrations. There was a disruption of mitochondrial membrane potential together with increased caspase-3 activity. Furthermore, findcution of apoptosis was confirmed by annexin V/PI analysis. The microarray gene profiling analysis revealed some important biological pathways including MAPK and inhibitor of DNA binding (ID) signaling pathways altered by fisetin and hesperetin treatment as well as gave a list of genes modulated involved in cell proliferation, cell division, and apoptosis. For CML cells, fisetin and hesperetin inhibited cell proliferation and triggered programmed cell death. The latter was confırmed by mitochondrial membrane depolarization and an increase in caspase-3 activation. In addition to that, we have detected S and G2/M cell cycle arrests and G0/G1 arrest upon fisetin and hesperetin treatment, respectively. The microarray gene profiling analysis revealed some altered important signaling pathways including JAK/STAT pathway, KIT receptor signaling. They both significantly modulated the expression of genes involved in cell proliferation and division, apoptosis, cell cycle regulation, and other significant cellular processes such as replication, transcription, and translation.

Email: [email protected]

image PDF   |   image HTML
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version