The presentation of the genes involved in iron or heme transport in R. anatipestifer through sequence comparison and analysis

2^nd International Conference on Genomics & Pharmacogenomics

September 08-10, 2014 DoubleTree by Hilton Hotel Raleigh-Brownstone-University, USA

Ma Feng Liu, Xing Jun Cheng, He Bin Liao, De Kang Zhu, Ming Shu Wang, Ren Yong Jia, Shu Chen and An Chun Cheng

Accepted Abstracts: J Mol Genet Med

Abstract :

Riemerella anatipestifer (R. anatipestifer, RA) is one of the most important bacterial pathogens that cause septicaemia and death in ducklings, and other avian species. It has been shown that R. anatipestifer encoded for iron/heme uptake systems and heme can be as an iron source. However, the mechanism of iron/heme uptake of R. anatipestifer and the proteins involved in iron/heme uptake have not been systematically studied. Unlike the organization of the iron/heme uptake system in other well studied gram-negative bacteria, the genes involved in iron/heme uptake are not in the same cluster in R. anatipestifer and were not been annotated well. Here, we presented and compared the iron/heme utilization related genes in four different R. anatipestifer genomes (RA-ATCC11845, RA-CH1, RA-CH2 and RA-GD), and the proteins functions of them in iron/heme uptake were predicted. Interestingly, we found that there exists a hemophore family protein and one hemophore-like protein (HmuY) in all sequenced R. anatipestifer genomes. In addition, we found five putative substrate-specific heme receptors and six siderophore receptors that could be involved in heme/hemoglobin and Fe3+-siderophore uptake, respectively. Accordingly, we also found the energy provider, one ExbB-ExbD-TonB, one ExbB-ExbDD-TonB and one TonB family protein to help the receptors transport substrate to periplasm. However, all these putative proteins have low similarity with other well-identified iron/heme transport proteins. Also, we can not find the gene encode for heme degrading protein through sequence analysis. It indicated that R. anatipestifer encode for a novel iron/heme uptake systems.

Biography :

Ma Feng Liu has completed his PhD at the age of 31 years from Paris VI University. He has published more than 12 papers in reputed journals.