Infertility is one of the consequence of chemotherapy in cancer patients due to its cytotoxic effect that induces
acute follicular damage, abnormal folliculogenesis leading to ovarian failure. Two crucial growth factors in abnormal
folliculogenesis namely Growth Differentiation Factor-9 (GDF-9) and Stem Cell Factor (SCF), which act on the oocyte-granulosa
cell interaction, will be disrupted and in turn it will affect follicular development. In this study we try to evaluate whether bone
marrow transplantation (BMT) has a role on oocyte-granulosa cell interaction by analyzing GDF-9 and SCF expressions and
also follicular development by analyzing primordial, primary, secondary and graafian follicles of experimental cisplatin-induced
ovarian failure in rat.
Animal laboratory experimental study
Materials and Methods:
Forty eight rats (rattus novergicus strain wistar) were divided into three groups : control, cisplatin and
cisplatin+BMT. Ovarian failure was induced by administration of intraperitoneal cisplatin dose 5 mg/kg body weight for 1
week. BMT 2x107 cell was injected through rat tail vein after cisplatin administration. Bone marrow was isolated from rat femur
6-12 weeks of age and characterized by CD44(+), CD45(-), CD105(+). Immunohistochemistry examinations for ovarian GDF-9,
SCF and follicle development evaluation were performed after 2 weeks of BMT injection. All three groups datas were compared
using the Anova test.
The expressions of GDF-9 (15.91 ? 0.69) and SCF (20.26 ? 1.14) in cisplatin+BMT group were higher than those in
cisplatin group: (5.33 ? 1.76) and (12.27 ? 2.88) and control group: (14.53 ? 1.42) and (20.22 ? 2,14) (p=0.000).
In cisplatin+BMT group the number of primordial (5.31 ? 1.30), primary (4.37 ? 0.88), secondary (3.62 ? 0.71) and graafian
follicles (2.75 ? 0.85) were higher than those in cisplatin group: (4.31 ? 1.19), (3.81 ? 1.22), (2.87 ? 0.95) and (0.37 ? 0.69); but
were lower than those in control group (6.12 ? 1.20), (4.93 ? 1.61), (4.25 ? 0.77) and (5.81 ? 1.37) (p=0.000).
Positive PKH labeling was seen in cisplatin+BMT group, while negative result in cisplatin group.
On cisplatin-induced ovarian failure in rat, bone marrow transplantation may improve oocyte-granulosa cell
interaction and follicular development. Further study is needed
Hendi Hendarto has completed his Ph.D in medical science (2007) from University of Airlangga Surabaya. He is supervisor of Graha Amerta IVF
Clinic at Dr Soetomo Hospital and also Head of Department of Obstetric and Gynecology University of Airlangga Surabaya, Indonesia
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