alexa The Role Of Cervical Mucus And Mucins In HIV/AIDS
ISSN 2155-6113

Journal of AIDS & Clinical Research
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

2nd International Conference on HIV/AIDS, STDs, & STIs
October 27-29, 2014 Embassy Suites Las Vegas, USA

Baxolele Mhlekude, Julia Peacock, Zoe Lots, Marylin Tyler, Delawir Kahn and Anwar Suleman Mall
Accepted Abstracts: J AIDS Clin Res
DOI: 10.4172/2155-6113.S1.009
Abstract
Introduction: Sexual intercourse is the major route of human immunodeficiency virus (HIV) transmission. Our laboratory reported that purified cervical mucins from HIV negative women inhibited HIV-1, whilst the crude cervical mucus did not. The anti-HIV-1 activity of crude cervical mucus and purified mucins from HIV positive women is unknown. We aimed to compare the anti- HIV-1 activities of the crude cervical mucus and purified mucins from HIV negative and HIV positive women in a large cohort. Methods: We collected cervical mucus plugs in guanidinium hydrochloride from fifteen HIV negative and eight HIV positive pregnant women, and stirred them overnight to solubilise. We subjected the samples to a low speed centrifugation to remove insoluble material and prepare crude cervical mucus. To purify mucins, we centrifuged samples twice by caesium chloride density gradient ultra-centrifugation, dialyzed against distilled water to desalt and freeze-dried to remove water. We confirmed the purity of mucins by SDS-PAGE and detected mucins by Western blot. We incubated separately the crude cervical mucus and purified mucins with HIV-1 for 48 hours, transferred each mixture to TZM-bl cells and detected the HIV-1 neutralization by illuminometer. Results: The mucin purification profiles showed that caesium chloride density gradient ultra-centrifugation removed protein contaminants from the mucins, as confirmed by the faint protein background on the SDS-PAGE. We detected MUC5AC and MUC5B in all samples using Western blot. We found that the crude cervical mucus and purified mucins from HIV negative and HIV positive women inhibited HIV-1 in an in vitro assay, with inter-individual variation among patients from both groups. Conclusion: Our findings suggest that HIV infection does not compromise the expression of the cervical mucins, and both crude cervical mucus and purified mucins can neutralize HIV-1 in an in vitro assay regardless the HIV status of the participants.
Biography
Baxolele Mhlekude has completed his BSc (Med) (Hons) by 2012 from the University of Cape Town, specializing in Medical Biochemistry and is now completing his MSc (Med) in the same institution and field. His MSc research is based on the anti-HIV-1 activities of the human cervical mucins and their ability to protect the growing foetus in the cervix during pregnancy against infectious agents, which usually ascend from the bacterial rich vaginal environment.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords