alexa The Role Of MiR-155 In The Apoptosis Of Human Lymphoma Cell Induced By CIK Cells
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
Open Access

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3rd International Conference and Exhibition on Clinical & Cellular Immunology
September 29-October 01, 2014 DoubleTree by Hilton Baltimore-BWI Airport, USA

Jiang Yi
Accepted Abstracts: J Clin Cell Immunol
DOI: 10.4172/2155-9899.S1.019
Abstract
Objective: To observe the effect of cytokine-induced killer cells (CIK) on the apoptosis of human lymphoma cells (Raji and BJAB), and explore the role of micro RNA-155(miR-155) in this process. Methods: MiR-155 was determined by real time quantitative PCR and the apoptosis was detected by flow cytometry in Raji and BJAB cells. Psi CHECK2-CEBPB 3?-UTR containing the binding site of miR-155 was constructed, and then transfected into Raji and BJAB cells. Luciferase activity of CEBPB (CCAAT/enhancer binding protein beta) was determined with the assistance of dual luciferase report system. Results: CIK cells could promote Raji and BJAB cells apoptosis (t=3.634, P<0.05; t=3.976, P<0.05), and increase the expression of miR-155 in Raji by (6.87?0.19) fold (t=2.787, P<0.05), meanwhile, in BJAB cells by (5.06?0.25) fold (t=3.513, P<0.05). Moreover, miR-155 inhibitor might block this process (t=3.842, P<0.05; t=4.016, P<0.05). Furthermore, miR-155 mimics induced Raji and BJAB cells apoptosis (t=4.239, P<0.05; t=3.477, P<0.05). MiR-155 targeted at the site of CEBPB 3?-UTR, and CIK cells could decrease the luciferase activity of Raji cells by (42.89?2.06)% (t=3.281, P<0.05); meanwhile, in BJAB cells by (37.02?1.98)% (t=4.933, P<0.05). Conclusion: CIK cells could enhance human lymphoma Raji and BJAB cells apoptosis by upregulating miR-155, which may provide a new database to elucidate lymphoma cell therapy using CIK cells.
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