alexa The Role Of Periostin In Regulation Of Early Tumorgenesis In Oral Squamous Cell Carcinomas | 13700
ISSN: 2161-1009

Biochemistry & Analytical Biochemistry
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

International Conference and Exhibition on Biochemical & Molecular Engineering

Gihan E. H. Gawish and Husein A. Alomer
Accepted Abstracts: Biochem Anal Biochem
DOI: 10.4172/2161-1009.S1.003
Abstract
Oral Squamous cell carcinomas are the most frequent malignancy of the oral cavity. Tissue Microarray analysis of OSCC revealed the up-regulation of POSTN gene expression in OSCC compared to normal tissues. It was reported that periostin contributes to malignancies mainly by preventing apoptosis and promoting angiogenesis, invasion, and metastasis. The roles of periostin in regulating cell proliferation, and genomic instability of cancer cells during tumorigenesis still require further investigation. The aims of this study was to indicate the oncogenic activity effect of periostin in OSCC, contribution of periostin in promoted OSCC proliferation and calculate the DNA index of ploidy and anploidy channels. SCC4, SCC9, SCC15, and SCC25 were transfected with periostin. For unbiased, siRNA and transfected Ca9-22 cells were used. Expression of periostin was analyzed then cells were stained by annexin V for apoptosis detection and PI for proliferation and DNA ploidy then stained cells were analyzed using FACScaliber. This study found that the oral squamous cell carcinoma cell lines transfected with periostin, exhibited significantly increased proliferation, compared with non-transfected. Periostin-overexpressing cells stained with annexin V and PI showed significantly (p<0.05) and (p<0.005) respectively. We have recorded that the number of hyperdiploid passages was 33% and 67% were diploid. Hyperdiploid passages with DNA index ≥1.16 and ≤1.6 were12 passages and hyperdiploid with DNA index >1.6 (11passages). We conclude that periostin play an important role in the tumorigenesis of OSCC by deregulation of the cell cycle, escaping from apoptosis, and the potential for unlimited replication. It encourages genetic alterations by enhancing chromosomal instabilities.
Biography

Gihan E. H. Gawish is an Assistant Professor Molecular Genetics in Medical Biochemistry Department, College of Medicine of Al-Imam University, Riyadh, Saudi Arabia. Also, She is a medical laboratory specialist since 2001. She earned her M.Sc. and Ph.D. at Mansoura University, Egypt. She is a Postdoc fellow, Oral Biological and Medical Science, University of British Columbia, Vancouver, Canada since 2011. She has published 12 peer-reviewed papers. Her research combines medical molecular genetics and advanced applications of techniques. Her research is based on detecting the signals that affect the tumorgenesis and introduce these signals with modified protocols to be investigated using modern current routine equipments in clinical laboratories for aiding in the early diagnosis and development of new personalized treatment approaches. She has been included in Marquis Whos who in the world 2013.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7