Journal of Microbial & Biochemical Technology

The study of genetic relationship among third generation cephalosporin-resistant Salmonella enterica strains

World Congress and Expo on Applied Microbiology

August 18-20, 2015 Frankfurt, Germany

Reza Ranjbar, Ali Najafi, Nadia Jahandeh, Nematollah Nonaidi and Morteza Izadi

Baqiyatallah University of Medical Sciences, Iran

Posters-Accepted Abstracts: J Microb Biochem Technol

Abstract:

Salmonella is an important food-borne pathogen responsible for disease in humans and animals. The aim of this study was to investigate the genetic relationship among third generation cephalosporin-resistant Salmonella enterica strains by Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR. The study included all Salmonella isolates obtained from clinical cases in a pediatric hospital in Tehran, Iran during 2006 to 2009. Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute. The genetic relationship between third generation cephalosporins-resistant Salmonella enterica strains was determined using ERIC-PCR. Of all Salmonella enterica isolates recovered from pediatric patients, six isolates including four Salmonella enterica serotype Infantis and two Salmonella enterica serotype Enteritidis showed an extended-spectrum cephalosporins resistant phenotype. ERIC-PCR differentiated Salmonella enterica serotypes Infantis and Enteritidis into 2 distinct clusters arbitrarily named as E1 and E2. Profile E1 was found in two Salmonella enterica serotype Enteritidis isolates, and profile E2 was found in four Salmonella enterica serotype Infantis isolates. Extended-spectrum cephalosporins resistant Salmonella could be attributed to a few predominant serotypes including Enteritidis and Infantis in this study. Genetic analysis using ERIC-PCR showed that closely related clones are responsible for the occurrence of extendedspectrum cephalosporins resistant Salmonella infection in Tehran.