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The development of a metabolomics platform workflow is hypothesis driven. The workflow is aimed at the development
of a sampling and extraction methodology, targeted quantification of metabolites via LC-MS/MS, and constraint-based
modeling approach using a genome-scale reconstruction to better understand and further improve metabolically engineered
and adaptively evolved strains of
. Specifically, progress in construction of a rapid sampling apparatus for batch-cultures
of anaerobic and aerobic strains, selection of isotope standards for targeted analysis based upon simulations of bacterial
growth, and testing of different sampling and extraction procedures will be described. Progress in the development of the
computational framework to analyze metabolomics data in the context of a genome-scale reconstruction of
to arrive at
biologically meaningful conclusions will also be presented.
Douglas Taylor is currently pursuing his Ph.D. at the University of California, San Diego in Bioengineering in the Systems Biology Research Group
under Professor Bernhard Palsson. Douglas Taylor earned his B.S. in biomedical engineering with a minor in material science with magna cum
laude honors at the University of California, Irvine. He has published (first author, Lab on a Chip, 2010, 10, 2472-2475; second author, Lab on a Chip,
2010, 10, 1623-1626) on the topic of fabricating low-cost microfluidic devices from shrink polymers. He is interested in incorporating metabolomics
data into constraint-based modeling for improved bacterial strain design.
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