alexa To A Safe Production - Steps In UHPLC Modeling
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

3rd International Conference and Exhibition on Advances in Chromatography & HPLC Techniques
July 13-14, 2017 Berlin, Germany

Imre Molnar and Rieger H J
Institute for Applied Chromatography, Germany
ScientificTracks Abstracts: J Chromatogr Sep Tech
DOI: 10.4172/2157-7064-C1-028
Abstract
In UHPLC method development, our goal is to achieve a satisfactory separation in less than one day. To do this, we use the software DryLab. We are starting with 2 gradient runs with a difference of factor 3 in their slope, tG1 and tG2. We run these 2 runs also at 2 temperatures T1 and T2 of 30°C difference. The resulting tG-T-model is the most successful design in UHPLC. To create a “Cube”, we repeat our tG-T-model for polar compounds at 3 different pH-values. For neutral substances, we vary 3 different organic eluents (MeOH, ACN and 50:50) to obtain selectivity changes (peak movements), which help us to find the best separation. The next step is Peak Tracking, which means to align a peak in a peak table in a horizontal line. Here we use peak areas to locate a peak in up to 12 runs. Further support in identification is the use of molecular masses and different UV-wavelengths. After peak tracking is finished, the model (the “Cube”) is calculated based on the so called “critical resolution” with more than 106 different chromatograms. The best separation can be found with one mouse click. However one separation is not telling you, how robust your method will be. Therefore we calculate the influence of tolerance limits for each parameter by producing for 6 factors at 3 levels (3^6=729) virtual experiments and calculate the success rate from them. This enables us to avoid “Out of specification” results, which are objects of a tedious “Change management” procedure, involving regulatory oversight and high costs. Finally we write a Knowledge Management Document, which includes the details of the above process, the input parameters, the input experiments, the graphical output of the Design Space and all necessary elements for a commercial authorization of the product by the regulatory agencies like the FDA, etc.
Biography

Imre Molnár studied Analytical Chemistry in Germany and was working with Csaba Horváth as a Post-doc fellow at Yale University in New Haven, CT, USA. After returning to Europe in 1981, he started working at the Institute for Applied Chromatography in Berlin. He is an HPLC method Development Expert and a pioneer of Modern Separation Modeling. Since 1986, he has been working with L R Snyder and J W Dolan on the development of the DryLab Software, which is used successfully worldwide in the pharmaceutical, chemical and life science industries for modeling and visualizing HPLC separations.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords