Journal of Aquaculture Research & Development

Transcriptomic and proteomic analysis of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp. salmonicida

International Conference on Aquaculture & Fisheries

July 20-22, 2015 Brisbane, Australia

Aihua Li1, Meng Long1, Juan Zhao2, Tongtong Li, Carolina Tafalla3, Qianqian Zhang1, Xiehao Wang1, Xiaoning Gong1 and Zhixin Shen2

Posters-Accepted Abstracts: J Aquac Res Development

Abstract:

Furunculosis caused by Aeromonas salmonicida subsp. salmonicida is an epidemic disease among salmonids including rainbow trout (Oncorhynchus mykiss). However, the immune mechanisms that are elicited in rainbow trout against the invasion of A. salmonicida are not yet fully understood. In this study, we examined the spleen to investigate the immune response of rainbow trout at 3d post infection by A. salmonicida at the transcriptome and proteome levels by using Illumina-seq and iTRAQ methods, respectively. A total of 1036 genes and 133 proteins were found to undergo differential expression during the immune response of the spleen against A. salmonicida infection. Gene ontology and KEGG analysis were conducted among the differentially expressed genes and proteins revealing that immune system process and response to stimulus were the top two biological processes and immune system, signaling molecules and interaction and immune diseases were the differential pathways activated. Correlation analysis of transcriptomic and proteomic results showed 17 proteins (11 up-regulated and 6 down-regulated) having consistent expression at RNA and protein levels. Moreover, protein?protein interaction analysis showed that diseases, proteasome, aminoacyltRNA biosynthesis and nucleotide metabolism were the main interactions among the consistently expressed proteins. Consequently, these up-regulated proteins namely ferritin, CD209, IL13R?1, VDAC2, GIMAP7, PSMA1 and two ANXA11s could be considered as potential biomarkers for rainbow trout immune responses.

Biography :

Aihua Li is currently a Professor in Institute of Hydrobiology, Chinese Academy of Sciences, China.