Transplantation of hepatocyte like cells derived from human tooth into cirrhosis rat model

7^th International Conference on Tissue Engineering & Regenerative Medicine

October 02-04, 2017 Barcelona, Spain

Yaegaki K

Nippon Dental University, Japan

Keynote: J Tissue Sci Eng

Abstract :

We treated the liver cirrhosis by transplantation of hepatocyte-like-cells derived stem cells from human exfoliated deciduous tooth (SHED) into the cirrhosis model. SHED at three passage was separated by magnetic sorting with CD117 antibody. For hepatic differentiation, CD117+ SHED were grown in DMEM supplemented with insulin-transferrinselenium-x (ITS-x), embryo-tropic-factors(ETF) and hepatocyte-growth-factor (HGF) for 5days: IMDM supplemented with ITS-x, ETF, HGF, dexamethasone and oncostatin for another 11 days. F344-nude rats were employed for this study. Carbon tetrachloride (CCl4) was administered by intraperitoneal injection for 12 weeks to induce cirrhosis. Hepatocyte-like-cells (2 x 106 cells/ animal) suspended in Hank`s Balanced Salt Solution were transplanted into the spleen. The vehicle was injected into the positive control group. Non-cirrhosis-models were used as negative control group. Animals were sacrificed 4 weeks after the transplantation.Immunocytochemistry observation of the hepatically differentiated cells strongly demonstrated positive staining for albumin, IGF-1, �±-feto-protein, HNF4�± and CPS-1. The histopathological analysis, HE and Massonâ��s trichrome staining, indicated a significant decrease of fibrous tissue in the transplantation group with comparison to the positive control group. Healthy liver tissues were recovered by the transplantation. Moreover, serological test results revealed significant differences between the groups. Serum ALT levels of the test group dramatically decreased to one-third compared to the positive control group. Activities of albumin, bilirubin, BUN, HA levels were also recovered. The xenotransplantation of hepatocyte-like cells from human tooth, transplanted into the liver with severe failure demonstrated their capacity to preform positively because of drastic decreasing fibrous tissues. Together, these findings suggested. Hepatocyte-like cells derived from SHED are a potential source for treating chronic liver injuries such as cirrhosis.

Biography :

Yaegaki K had received Doctor of Dental Science, and then completed his PhD in Medicine from Kureme University Medical Biochemistry and Postdoctoral studies from University of British Columbia. He was trained as an Oral Maxillofacial Surgeon at School of Medicine Kurume University. His speciallity was facial injuries. He is the Director and Head of Oral Health at Nippon Dental University, which is the oldest Dental School in Asia, also a Dean of PhD program. He has published more than 100 papers and 20 books.