alexa Treatment Of Soil With L-alanine And Inosine As Germinants May Drop The Number Of Living Cells Of Bacillus Anthracis Below An Infectious Dose Level | 23410
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

2nd International Congress on Bacteriology & Infectious Diseases
November 17-19, 2014 DoubleTree by Hilton Hotel Chicago-North Shore, USA

Benedict Ntui and Wolfgang Beyer
Accepted Abstracts: J Bacteriol Parasitol
DOI: 10.4172/2155-9597.S1.008
Abstract
Introduction: Spores of B. anthracis are highly resistant to adverse environmental conditions and chemical disinfectants. Reducing the soil spore count below a certain risk level can be highly desirable. The synergistic effect of spore germinants and chemical disinfectants have shown to significantly reduce spore counts in soil in laboratory based soil microcosm experiments. Here we investigated the effect of germinants alone on spore reduction. Methods: 200 g of commercial potting soil (Universal potting mix) and Natural soil (Mature compost from Hohenheim garden wastes) was spiked with 200 ml containing spores of the Sterne strain with and without a GFP label, concentrated at 1.6x106 spores/ml, and dried overnight at 40?C. 42 g of the spiked soil was treated with 20 ml of 100 mM L-Alanine and 5 mM inosine. Another 42 g of the spiked soil was treated with 20 ml of sterile deionised water as a control. Samples were incubated both at 37?C and at 4?C. 2 g from each soil type were monitored in triplicate after 1, 2, 6 hours and 1, 2, 5 and 14 days, with and without heat treatment at 65?C for 30 min. Results: 90% reduction of heat resistant spores was found after 1 hour at 37?C incubation temperature, while the total cell count remained stable. After 1 day at 37?C, both total cell and heat resistant spore counts were reduced by ≥99.9%. At 4?C the total cell counts and heat resistance spores reduced by >1 log10 and ≥2 log10 respectively only after 14 days. In the control the number of spores remained steadily at spike level during the entire period. Microscopic observations of gfp labeled spores revealed a complete germination of a great majority of spores with single, non- replicating vegetative cells at 37?C after 24 hours and at 4?C after 14 days. Conclusion: The results indicate that the use of germinants alone can significantly reduce the number of living B. anthracis in soils independently on the soil type. However, the role of indigenous soil microflora on the fate of the germinating spores and vegetative cells in these soils needs further to be investigated.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version