Trimethoprim-sulfamethoxazole Resistance In Clinical Isolates Of E. Coli From UTI Patients In Zaria, Nigeria | 58870
Journal of Medical Microbiology & Diagnosis
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The increasing trend of E. coli resistance to trimethoprim-sulfamethoxazole (TMP-SMZ) among UTI patients is indeed becoming
worrisome, as this first class antibiotic of hope in the last few years is becoming ineffective due to resistance associated with
emerging and reoccurrence of E. coli infections in UTIs. This study evaluates the resistance pattern of TMP-SMZ resistant E. coli
from UTI patients in Zaria, Nigeria using standard microbiological methods. The result showed that out of the 80 suspected E. coli
isolates from UTI patients in 4 selected hospitals in Zaria, 68.75% (55) of the isolates were confirmed as E. coli using Macrobat 12E
Enterobacteriacea identification kit. Out of which 58.2% (32) were resistant to TMP-SMZ. High resistance profile of the isolates
were observed against amoxicillin and cefpirome (96.9%), followed by ceftaxime (78.1), tetracycline (75%), cefpodoxime (68.8),
ciprofloxacine and ofloxacin (59.4), chloramphenicol (53.1%), gentamicin (46.9%), aztreonam (31.3%), nitrofurantoin (28.1%) and
cetriaxone (21.9%), the most effective antibiotics were observed to be amikacin (3.1) and imipenem (0%). The isolates were observed
to have 100% MDR at MARI≥0.2. Molecular analysis using PCR showed that dfrXII gene was present in all the 21 (100%) MDR E. coli
isolates, 85.7% (18) had sul2 while dfrIa and sul1 genes were absent in all the isolates evaluated in this study. This study showed that
high percentage of clinical isolates of E. coli was resistant to TMP-SMZ in Zaria metropolis. Hence, there is the need to re-strategize
on the surveillance approach, awareness programmes on antibiotics resistance and dispensing of antibiotics in clinics and community
pharmacy in other to curb the resistant trend of bacteria to wide antibacterial spectrum like trimethoprim-sulfamethoxazole (TMPSMZ)
among UTI patients in Nigeria.
Igwe James Chibueze has completed his PhD in Pharmaceutical Microbiology, MSc in Pharmaceutical Microbiology from Ahmadu Bello University, Zaria, Nigeria) and BSc in Biotechnology from Ebonyi State University, Abakaliki, Ebonyi State. He is currently working as Scientific Officer I (Research Scientist) at the Department of Medical Biotechnology, National Biotechnology Development Agency, Abuja, Nigeria.