alexa Tumorspheres From In Vitro Transformed Cell Lines Show Molecular Signatures Related To Stemness And Transformation
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

10th International Conference on CANCER STEM CELLS AND ONCOLOGY RESEARCH
June 26-28, 2017 London, UK

Chiara Mondello
Institute of Molecular Genetics, Italy
Keynote: J Cancer Sci Ther
DOI: 10.4172/1948-5956-C1-101
Evidence indicates that a subset of cells endowed with high tumorigenic potential and stemness features (cancer stem cells: CSCs) is responsible for tumor initiation and maintenance in several cancers. In this study, we used a tumor cellular model developed in our laboratory from telomerase immortalized human fibroblasts (named cen3tel) and the tumorsphere assay to possibly isolate and characterize CSCs from in vitro transformed cells. We found that cen3tel cells were able to form spheres (frequency ~ 2-10%) and sphere cells showed self renewal capacity and Sox2 overexpression, suggesting that in these populations there is a subset of cells with CSC-like features. The characterization of sphere cells revealed that they displayed the activation of pro-survival mechanisms, through the expression modulation of genes as c-MYC, GNL3 and Notch, and the tumor suppressor miR-34a, which could favor the growth of cells in suspension upon detachment from a solid support. Moreover, genome wide gene expression profiles of sphere cells relatively to adherent cells revealed an extensive transcriptional reprogramming involving several stemness and cancer-related genes, indicating that transformed cells are highly plastic entities adopting specific gene expression programs depending on different environmental conditions. However, sphere cells were only slightly more tumorigenic in vivo than adherent cells suggesting that different subpopulations can support tumorigenicity in transformed cells, highlighting a further level of complexity in tumor heterogeneity.

Chiara Mondello is Senior Researcher and Group Leader at the Institute of Molecular Genetics of the National Research Council in Pavia (Italy). Her main interests concern the study of genome instability and cellular transformation in mammalian cells. She has published more than 80 papers in peer-reviewed journals and several chapters in books. She has been the Editor of the book “Multiple Pathways in Cancer Development” (Transwell Research Network).


image PDF   |   image HTML

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version