alexa Typing Of Mycobacterium Tuberculosis Strains By DNA Fingerprinting Methods (RAPD, ERIC AND GTG-5 PCR)
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

2nd International Congress on Bacteriology & Infectious Diseases
November 17-19, 2014 DoubleTree by Hilton Hotel Chicago-North Shore, USA

Golnar Sami Kashkooli
Accepted Abstracts: J Bacteriol Parasitol
DOI: 10.4172/2155-9597.S1.008
Abstract
Background: Application of molecular methods in order to identification and isolation of Mycobacterium tuberculosis complex from Non tuberculosis Mycobacteria is useful for appropriate treatment of tubercular patients and interruption of transmission chain in endemic areas. This study aimed to assess the PCR-RFLP method for simultaneously identification and isolation of Mycobacterium tuberculosis complex of Non tuberculosis Mycobacteria and typing of M. tuberculosis strains by PCR-based fingerprinting methods. Methods: This cross - sectional, descriptive study of 120 mycobacterial strains isolated from the Lowenstein ? Jenson Media, referring to patients from Fars province and neighboring provinces was conducted in 1391. After differential biochemical tests, PCR-RFLP on hsp65 gene was performed. Mycobacterium tuberculosis strains then subjected to fingerprinting techniques (RAPD, ERIC, GTG5) to find heterogeneity of them. Results: Of the 120 cases reviewed, 112 cases (93/44%) were M. tuberculosis from aspect of yielded pattern of generated bands on polyacrylamide gel and 8 cases (6/66%) belonged to the group of non tuberculous mycobacteria (3 M. chelonaeand 5 M.gordonae). By usage of fingerprinting techniques, different bands and profiles polymorphism among strains were obtained. RAPD method using primer INS-2, revealed the highest degree of genetic diversity among the three fingerprinting techniques. Conclusion: The results showed that the RFLP-PCR, is quick, easy method with high sensitivity and specificity which generate unique patterns in Mycobacterium species. Detection of species leads to proper decision for treatment .between typing methods, (RAPD) showed more diversity. Therefore utilization of this method with other molecular typing methods is recommended in order to identification and clustering of strains for epidemiological survey.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords