alexa UHPLC-MS/MS Analysis Of Thyreostats In Serum
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th World Congress on Chromatography
August 07-09, 2017 | Rome, Italy

Sebastian Witek, Barbara Wozniak, Iwona Matraszek-Zuchowska and Andrzej Posyniak
National Veterinary Research Institute, Poland
Posters & Accepted Abstracts: J Chromatogr Sep Tech
DOI: 10.4172/2157-7064-C1-032
Abstract
Thyreostats are thioamide antithyroid drugs. Activity of these compounds consists in inhibiting the synthesis of thyroid hormones triiodothyronine (T3) and thyroxine (T4), which favors the processes of animal fattening. Increase in weight of animals is mainly due to the water retention in the tissues and the gastrointestinal tract. The consequence is not only the production of lower meat quality, but also the risk of drug residues to human health. According to the International Agency for Research on Cancer, some compounds of this group possess carcinogenic and teratogenic properties. In accordance with the Council Directive 96/23/EC thyreostats belong to the group A2 - compounds with anabolic properties, which must be controlled on slaughter animals. To extend the range of methods used in the national control plan, a fast and efficient UHPLCMS/ MS method was developed and validated to detect five thyreostatic compounds: tapazole, thiouracil, methylthiouracil, propylthiouracil and phenylthiouracil in bovine serum. Previously published method of thyreostats in urine was used as a starting point for the development of our method. Thyreostats were extracted from serum samples with diethyl ether after derivatization with 3-iodobenzylbromide in basic medium (pH 8.0) and analyzed by gradient elution within 7.5 min on a SBC18 column (50 x 2.1 mm; 1.8 μm, Agilent) using a mobile phase consisting of acetonitrile/0.1% acetic acid. The analysis was performed on UHPLC Shimadzu NEXERA X2 with triple quadrupole MS 8050 instrument operating in positive electrospray ionization mode. The procedure was validated according to the Commission Decision 2002/657/EC requirements. The recovery and repeatability satisfy the performance criteria specified in this document for banned compounds. The recovery ranged from 97.5% to 113.5% for all examined compounds, and the repeatability did not exceed of 14.1%. The decision limits (CCα) did not exceed 3.95 μg L-1, and also detection capabilities (CCβ) did not exceed 6.73 μg L-1. The developed procedure is sensitive and robust, and therefore, useful for quantification and confirmation of thyreostats in residue control programme.
Biography

Sebastian Witek works at National Veterinary Research Institute, Poland since 2009. He deals with residues of hormones and thyreostats in biological samples of animal origin. He has a lot of experience in LC-MS/MS and GC-MS.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords