alexa Ultra-performance Liquid Chromatography-mass Spectrometry, A Tool To Estimate The Treatment Effect Of The Water Extract Of Polygonum Cuspidatum (Hu Zhang) In Diet-induced Hypercholesterolemic Rats
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

3rd International Conference and Exhibition on Advances in Chromatography & HPLC Techniques
July 13-14, 2017 Berlin, Germany

Shun-Wan Chan, H uan Zhang, Tung-Ting Sham, Yam-Fung Ng, Tsun-Ka Kwok, Chi-Lui Rose Wong, Hei-Man Tang, Chi-On Chan and Daniel Kam- Wah Mok
The Hong Kong Polytechnic University, Hong Kong
State Key Laboratory of Chinese Medicine and Molecular Pharmacology, China
Technological and Higher Education Institute of Hong Kong, Hong Kong
Posters & Accepted Abstracts: J Chromatogr Sep Tech
DOI: 10.4172/2157-7064-C1-029
Abstract
The dried roots of Polygonum cuspidatum Sieb. et Zucc. (HZ, Hu Zhang in Chinese) have been used for the treatment of hepatitis, scald and hyperlipidemia in oriental counties. However, the effects of water extract of HZ on lipid-lowering activity still remains in a black box due to lack of appropriate methods. The present investigations described the major components of HZ water extract using high-performance liquid chromatography with diode-array detection (HPLC-DAD) and ultra-performance liquid chromatography with orbitrap mass spectrometry (UPLC-Orbitrap-MS). Additionally, the effects of HZ water extract on serum in rats fed with high fat diet (HFD) was analyzed using metabolomics approach by ultra-performance liquid chromatography quadrupole time of flightmass spectrometry (UPLC-QTOF-MS). The major components of HZ water extract have been identified as polydatin, emodin, resveratrol, emodin 8-O-β-D-glucopyranoside, physcion O-β-D-glucopyranoside and piceatannol (Fig. 1). Data from serum analysis showed that the supplementation of HZ extract significantly lowered the levels of total cholesterols. Multivariate statistics revealed that treatment of HZ significantly elevated serum levels of chenodeoxycholate, deoxycholate, their glycine-conjugated bile acids and glycocholate level when compared with HFD model, indicating that the underlying cholesterol-lowering mechanism of HZ might be associated with bile acid biosynthesis. In order to verify the mechanism, bile acid biosynthesis related proteins, including cytochrome P450 7A1(CYP7A1) and mitogen-activated protein kinase (MAPK) [P38, extracellular regulated protein kinase (ERK) and c-Jun N-terminal kinase (JNK)] pathway were investigated using Western blot assay. The expressions of these proteins were found to be upregulated by HZ treatment, supporting that the cholesterol-lowering effect of HZ is associated with bile acid metabolisms.
Biography

Shun-Wan Chan is an Associate Professor in the Department of Food and Health Sciences, Faculty of Science and Technology, Technological and Higher Education Institute of Hong Kong. His research is focused on studying the molecular mechanisms of functional foods on preventing chronic diseases, such as cardiovascular diseases and diabetes mellitus. His research interests in food science also include food safety, food microbiology and toxicology.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]com

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords