alexa UPLC-MS Method For The Simultaneous Determination Of Phenolic Constituents In Honey Using Multiwalled Carbon Nanotubes As Solid Phase Sorbents
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th World Congress on MASS SPECTROMETRY
June 19-21, 2017 London, UK

Zeid Abdullah Alothman
King Saud University, Saudi Arabia
Posters & Accepted Abstracts: J Chromatogr Sep Tech
DOI: 10.4172/2157-7064-C1-026
Abstract
An ultra-performance liquid chromatography-tandem mass spectrometer (UPLC-MS) method has been developed for the simultaneous separation, identification and determination of 22 phenolic constituents in honey from various floral sources from Yemen. Solid-phase extraction was used for extraction of the target phenolic constituents from honey samples, while multi-walled carbon nanotubes were used as solid phase adsorbent. The chromatographic separation of all phenolic constituents was performed on a BEH C18 column using a linear gradient elution with a binary mobile phase mixture of aqueous 0.1% formic acid and methanol. The quantitation was carried out in selected ion reaction monitoring acquisition mode. The total amount of phenolic acids, flavonoids and other phenols in each analyzed honey was found in the range of 338-3312, 122-5482, and 2.4-1342 μg/100 g of honey, respectively. 4-hydroxybenzoic acid was found to be the major phenolic acid. The main detected flavonoid was chrysin, while cinnamic acid was found to be the major other phenol compound. The regeneration of solid phase adsorbent to be reused and recovery results confirm that the proposed method could be potentially used for the routine analysis of phenolic constituents in honey extract.
Biography

Zeid Abdullah Alothman is working as a Professor in Department of Chemistry at King Saud University, Riyadh, Saudi Arabia. He completed his BSc in 1997 at King Saud University and PhD in 2006 at Oklahoma State University, Stillwater, USA. His research has been focused on “The application of chromatography separation methods in environmental, chemical and pharmaceutical studies, safe pollutants removal, and synthesis of new silica based materials for separation (packing materials for chromatographic columns)”. He has guided few PhD and masters students and published book chapter in the various field of Chemistry.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords