alexa Using Monoclonal Antibodies To Understand The Molecular Basis For The Cross Bactericidal Activity Of NHBA Antigen
ISSN: 2157-7560

Journal of Vaccines & Vaccination
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

4th International Conference on Vaccines & Vaccination
September 24-26, 2014 Valencia Convention Centre, Spain

Facciotti C, Bertoldi I, Galli B, Bartolini E, Donnarumma D, Lo Surdo, P Norais, N Santini, L Masignani and Giuliani M
Posters: J Vaccines Vaccin
DOI: 10.4172/2157-7560.S1.021
Abstract
T he Neisserial Heparin Binding Antigen (NHBA) is a protective antigen of N. meningitidis and is one of the components of the recently licensed vaccine Bexsero. NHBA was demonstrated to be an important virulence factor and to be able to elicit a robust immune response in humans against meningococcal strains expressing homologous and heterologous NHBA peptides. In order to better understand the cross protective nature of NHBA we performed a screening with the purpose to obtain monoclonal antibodies (mAbs) to be used as tools in the process of characterization. Mice were immunized with different forms of recombinant NHBA peptide 2, the same peptide present in the Bexsero formulation. When tested in the SBA assay, two of the new mAbs displayed bactericidal activity and other two mAbs were bacteriostatic against meningococcal strains expressing the homologous protein. Antibody binding and antibody affinity were measured on the recombinant protein by enzyme-linked immunosorbent assay (ELISA) and Surface Plasmon Resonance (SPR) experiments, respectively, whereas Fluorescence-activated cell sorter (FACS) analysis was used to detect the native protein on bacterial surface. Cross-bactericidal activity was assayed by testing the selected mAbs on a panel of MenB strains expressing different NHBA peptides. Finally, a plethora of epitope mapping techniques, including protein chip analysis on NHBA fragments and Hydrogen-Deuterium Exchange (HDX-MS) were adopted to precisely map the position of the recognized epitopes. By this approach we have mapped protective epitopes on NHBA and studied their possible role on NHBA cross bactericidal activity.
Biography
Facciotti C, Biologist has a PhD in Science and worked at Novartis Vaccines in Siena (Italy) since 2005 in the Protein Biochemistry Unit at the Research Center. She is specialized in purification of recombinant proteins from E. coli and their characterization. She has done analytical studies on purified proteins as SE_UPLC/ HPLC and RP_HPLC for protein integrity/purity and SCK for the protein/antibodies interactions by BiacoreT200.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

engineeringjournals[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version