alexa Validation Of A Fresh-frozen Tissue Based Prognostic Gene Signature In Formalin-fixed, Paraffin-embedded Melanomas
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

3rd World Congress on Cancer Science & Therapy
October 21-23, 2013 DoubleTree by Hilton Hotel San Francisco Airport, CA, USA

G. Brunner, A. Heinecke, L. Suter, C. Berking, H.-J. Schulze and J. Atzpodien
Accepted Abstracts: J Cancer Sci Ther
DOI: 10.4172/1948-5956.S1.030
Abstract
Histopathological staging of melanoma is limited in predicting outcome, and complementary molecular markers are not available for routine prognostic assessment. We have recently identified and validated a prognostic nine-gene signature expressed in fresh-frozen primary cutaneous melanomas (training cohort: n=91; validation cohort: n=44). A signature-based risk score predicts patient overall survival independently of AJCC staging (multivariate regression analysis: p=0.0004; hazard ratio: 3.8). The purpose of the present study was to establish signature expression analysis in formalin-fixed, paraffin-embedded (FFPE) melanomas to validate prognostic significance of the signature-based risk score.From FFPE melanomas matching the training and validation cohorts of the above study (n=125), RNA was prepared and transcribed into cDNA. Following cDNA pre-amplification, expression of the 9 signature genes, 2 additional candidate genes, and 3 housekeeping genes was quantified by real-time PCR. Correlation of gene expression with overall survival was evaluated using cox regression analysis. Expression of a signature of 8 out of 11 genes was significantly associated with overall survival in univariate cox regression analysis. A signature based risk-score predicted overall survival independently of AJCC staging (multivariate analysis: p=0.007, hazard ratio 3.0). Thus, the fresh-frozen tissue based prognostic gene signature was successfully validated in FFPE melanomas.We have established a quantitative, robust prognostic score, based on FFPE melanomas, that is complementary to AJCC in predicting outcome. This greatly increases clinical applicability and allows retrospective prognostic assessment of melanomas. The score identifies patients at low risk, not identified by AJCC staging, and defines high-risk patients in need of adjuvant therapy.
Biography
G. Brunner has completed his Ph.D. in Germany and postdoctoral studies at the German Cancer Research Center in Heidelberg. He spent more than 8 years abroad, as Research Assistant Professor at NYU and lecturer at Manchester University in the UK. He is the Head of Research at the Skin Cancer Center Hornheide-M?nster in Germany. He has published more than 50 papers in reputed journals and is inventor on 3 international patent
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords