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Whole cell bacterial biosensor for environmental monitoring and pollutants detection
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Biosensors & Bioelectronics

ISSN: 2155-6210

Open Access

Whole cell bacterial biosensor for environmental monitoring and pollutants detection


3rd International Conference and Exhibition on Biosensors & Bioelectronics

August 11-13, 2014 Hilton San Antonio Airport, San Antonio, USA

Saurabh Gupta

Scientific Tracks Abstracts: J Biosens Bioelectron

Abstract :

Whole-cell microbial sensors have become one of the latest approaches of molecular tools in environmental monitoring. A whole-cell bacterial biosensor capable of detecting a wide range of pollutants (eg- aromatic hydrocarbons) can be created by placing a reporter gene under the control of an inducible promoter. Expression of the reporter gene provides a measurable response when the appropriate transcription activator protein interacts with a pollutant molecule to signal a particular environmental condition. Luciferase is the most sensitive and preferred reporter gene in the application of environmental monitoring, which signals in the form of luminescence. The luminescence is produced by genetically engineered bacteria as a response to the analyte concentration. Linearity range of the curve is determined by exposing the whole cell biosensor to different concentration of analyte and measuring the output luminescence. In summary, this presentation is intended to develop improved bacterial whole-cell sensing systems for detection of water pollutants and environmentally relevant analyte like hydrocarbons, which is typically sensitive, specific and selective, rapid, easy to use, low-cost, and amenable to multiplexing, high-throughput, and miniaturization.

Biography :

Saurabh Gupta has completed his PhD from IGIB (Institute of Genomics and Integrative Biology, New Delhi) and pursued postdoctoral studies from ICGEB, New Delhi. Currently, he is working as a Young Scientist in CEERI, Pilani, India.

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Citations: 1751

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