Yellow Laccase Mediated Delignification: An Attempt Towards Eco-friendly Pretreatment Of Lignocellulosics | 44440
Journal of Fundamentals of Renewable Energy and Applications
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Ever-increasing energy demand in developed as well as in developing nations has prompted worldwide interest in the
production of biomass-based fuels as a substitute to petroleum fuels. At this juncture, biofuel production from biomass
represents a sustainable resource and a better alternative for achieving zero emissions. This in turn necessitates the development
of an efficient biomass delignification method, which is an essential prerequisite for the complete biofuel production process.
Lignocellulosics such as Kans grass, sugarcane top, pineapple leaf waste, Bambusa bambos, Ricinus communis , Lantana
camara and mixtrue contain 13-20% lignin and 60-70% carbohydrates within its cell walls. To make this enormous amount of
carbohydrates more accessible for hydrolysis during fermentation, lignin degradation by yellow laccase has been attempted. In
the present study, response surface methodology (RSM) based on central composite design (CCD) has been used to investigate
the effects of the various process parameters on biomass delignification. The maximum 80-85% delignification obtained for the
above mentioned lignocellulsics within 5-6 h. Thorough study of the raw and pretreated biomass were carried out by elemental
composition analyses and energy density measurement. Further structural characteristics of the enzymatically delignified
substrate were analyzed by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray
diffraction (XRD) spectroscopy and porosity analysis that supported the efficacy of the enzymatic delignification process.
Rintu Banerjee, Ex-MNRE-Chair-Professor, Indian Institute of Technology, Kharagpur has created a niche of her own in the area of Biomass Deconstruction/Biofuel Production/Enzyme Technology. In the process of her innovative development, she was granted 8 Indian, 3 International (US, Japanese and Chinese) patents. She has published more than 150 papers in peer-reviewed national/international journals and guided many PhD, MS, MTech and Btech students. She is the Editorial Member of many journals. She has written 24 book chapters and authored a book on “Environmental Biotechnology” published by Oxford University Press. She is recipient of various awards/honours given by both government/non-government organizations.