alexa Zebrafish Model Of Acute Kidney Injury Sheds New Light On Cyst Formation In ADPKD
ISSN: 2161-0959

Journal of Nephrology & Therapeutics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

12th Global Nephrologists Annual Meeting
June 26-28, 2017 London, UK

Aleksandr Vasilyev
New York Institute of Technology, USA
Keynote: J Nephrol Ther
DOI: 10.4172/2161-0959-C1-038
Studies of kidney development and repair after injury are difficult in mammals due to complex architecture of metanephric kidney and the inherent difficulty of in vivo imaging in mammals. Transparent zebrafish larvae have simple pronephric kidneys that closely resembles mammalian nephron in segment identity and arrangement. This vertebrate model allows direct in vivo visualization of kidney development and repair after injury. We developed a novel assay of Acute Kidney Injury (AKI) in GFP transgenic zebrafish larvae using violet laser ablation. This technique allows us to directly visualize the sub-cellular, cellular and organ-level interactions driving kidney injury and repair. For example, by using this technique, we showed that collective cell migration is the primary repair mechanism after AKI. It had been recently proposed that rapid cyst induction in conditional mouse models of ADPKD requires a “third hit”, and that AKI serves this role. The suggested interpretation of the interaction between AKI and cyst formation is that injury induces proliferative response in kidney epithelia, thus promoting cyst progression. By utilizing our novel zebrafish model of AKI we show that kidney obstruction and not increased cell proliferation is the driving force leading to rapid cyst initiation after injury. We also show that PKD2 mutant zebrafish demonstrate slower resolutuon of transient cysts after acute injury when compared to control siblings. This findings shed new light on the nature of the “third hit” suggested by inducible mouse models of ADPKD.

Alex Vasilyev received his PhD and MD degrees from Rosalind Franklin University of Medicine and Sciences. He then completed his Residency training in Anatomic Pathology at the Massachusetts General Hospital and a Research Fellowship in Renal Pathology. He became an Instructor in Pathology at the Massachusetts General Hospital/Harvard Medical School, and later, he started as an Assistant Professor in Biomedical Sciences at the New York Institute of Technology, College of Osteopathic Medicine. He studies kidney development, regeneration and pathophysiology using zebrafish as a model.

Email: [email protected]

image PDF   |   image HTML

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version