alexa Zinc/cPLA2 Associated Autophagy And CaMK II Pathway Contributed To Neurobehavioral Deficits And Hippocampal MFs Sprouting Following Neonatal Seizures And Its Intervention By Chronic Leptin Treatment | 60571
ISSN: 2167-0897

Journal of Neonatal Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

9th International Conference on Neonatology and Pediatric Neurology

Ni Hong
Children’s Hospital of Soochow University, China
ScientificTracks Abstracts: J Neonatal Biol
DOI: 10.4172/2167-0897.C1.002
Abstract
Because leptin was affecting expression of brain energy metabolism-related genes and because zinc/lipid metabolism signals and its associated autophagy stress were also found to be involved in energy depletion, we hypothesized that leptin might exert its neuroprotective action via zinc/lipid and autophagic metabolism signaling. Here, we tested this hypothesis by examining the long-term expression of zinc transporter ZnT1 and ZIP7, lipid membrane injury-related cPLA2, its downstream autophagy marker Beclin-1, LC3, bcl2 and Cathepsin-E, as well as its execution molecule CaMK II following neonatal seizures and chronic leptin treatment. On postnatal day 6 (P6), 40 Sprague-Dawley rats were randomly assigned to two groups: Flurothyl-induced recurrent seizures group and control group. On P13, they were further randomly divided into the seizure group without leptin (RS), seizure plus leptin (RS+Leptin, 2 mg/kg/day, consecutive 10 days), the control group without leptin (Control), and the control plus leptin (Leptin, 2 mg/kg/day, consecutive 10 days). Neurological behavioral parameters of brain damage (negative geotaxis reaction reflex, plane righting reflex, cliff avoidance reflex, forelimb suspension reflex and open field test) were observed from P23 to P30. Morris water maze test was performed during P27-P32. Then hippocampal mossy fiber sprouting and the protein levels were detected by Timm staining and Western blot analysis, respectively. Flurothyl-induced RS rats show a long-term lower amount of CaMK II alpha, and higher amount of ZnT1, ZIP7, cPLA2, Beclin-1/bcl2, LC3II/LC3I and Cathepsin-E which are in parallel with hippocampal mossy fiber sprouting and neurobehavioral and cognitive deficits. Furthermore, chronic leptin treatment (RS+Leptin) is effective in restoring these molecular, neuropathological and cognitive changes. The results imply that a zinc/lipid metabolism-associated membrane peroxidation and autophagy pathway is involved in the aberrant hippocampal mossy fiber sprouting and neurobehavioral deficits following neonatal seizures, which might be a potential target of leptin for the treatment of neonatal seizure-induced brain damage.
Biography

Ni Hong has completed his PhD from Zhejiang University and Post-doctoral studies from Peking University School of Medicine. He is the Director of Neurology Laboratory of Children's Hospital Affiliated to Soochow University and Deputy Director of the Institute for Pediatric Research, Soochow University.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7