Quantitative Estimation of Lopinavir and Ritonavir in Tablets by RP-HPLC Method

One of the deadliest and unmanageable chronic health catastrophes is HIV/AIDS. It requires lifelong treatment with potent life saving essential drugs that include nucleoside reverse transcriptase inhibitors, non nucleoside reverse transcriptase inhibitors and protease inhibitors. Amongst these lopinavir and ritonavir drug combination is a protease inhibitor used as a second line regimen to treat patients with HIV [1].


Introduction
One of the deadliest and unmanageable chronic health catastrophes is HIV/AIDS. It requires lifelong treatment with potent life saving essential drugs that include nucleoside reverse transcriptase inhibitors, non nucleoside reverse transcriptase inhibitors and protease inhibitors. Amongst these lopinavir and ritonavir drug combination is a protease inhibitor used as a second line regimen to treat patients with HIV [1].
The proposed method was optimized and validated in accordance with International Conference on Hormonization (ICH) guidelines [12]. The aim of present work is to develop a simple, rapid, precise, accurate and selective reversed phase chromatographic method and to estimate the lopinavir and ritoavir in bulk and its solid dosage forms.

Chemicals
The bulk drugs of lopinavir and ritonavir were obtained as gift samples from Abbott Laboratories Ltd, Guwahati, India. HPLC grade acetonitrile and ammonia were obtained from Sigma Aldrich (Switzerland). Combination tablets of Lopinavir 200 mg and Ritonavir 50 mg from abbott were purchased from local market. Milli-Q-Water was used in all experiments. All the solutions for analysis were prepared and analyzed freshly.

Instrumentation and analytical conditions
Chromatography was performed using a shimadzu LC-10ATvp series, (Kyoto, Japan) equipped with SPD-10A UV-Vis detector. Data acquisition and processing was performed using chemistry station software (LC solution). The methods were conducted using a gradient  Table 1. The mobile phases were prepared freshly, filtered through 0.45 µ membrane filter (Millipore, USA) and sonicated (Branson sonicator 3210, Germany) for 10 min before use in order to deaerate.

Preparation of standard and quality control solutions
Primary stock solutions of lopinavir (100 mg) and ritonavir (100 mg) were prepared in ultra pure water and further diluted with water to obtain working standards in the concentration range of 40-200 µg/ ml and 10-50 µg/ml for lopinavir and ritonavir respectively. Quality control (QC) samples were run with each batch of working standards in order to calculate the validation parameters. QC samples were prepared in ultra pure water spiked with analytes at different concentrations following the same procedure as for calibration standards, using a different primary stock. The samples were analyzed with reagent blanks. All the solutions were prepared in triplicates.

RP-HPLC method
A RP-HPLC method was developed for two anti-retroviral drugs, which can be conveniently employed for routine quality control in pharmaceutical dosage forms. The chromatographic conditions were optimized in order to provide a good performance of the assay. The mobile phase for each drug was selected based on its polarity. Different ratios of Buffer: ACN combinations were tried for lopinavir and ritonavir and the fixed mobile phase are listed in Table 1. The optimization of flow rate is critical since the extent of longitudinal broadening is inversely related to flow rate of mobile phase. In either case of high or low flow rates, an ideal Gaussian curve of the peak is not obtained as the peak symmetry parameters are affected, i.e. asymmetry factor deviates from unity (Figure 2-4). The retention times of ritonavir and lopinavir were 4.323 and 5.650 min, respectively. The chromatograms have been shown in Figure 5. The methods were specific as none of the excipients interfered with the analytes of interest. Hence, the methods were suitably employed for assaying the commercial anti-retroviral individual formulations.

Linearity
Calibration curves were obtained from the peak area and concentration of the drug were subjected to regression analysis and correlation coefficients. Table 2 represents the mean RP-HPLC area responses for ritonavir and lopinavir at different concentrations. As shown, the responses for the drug was strictly linear (r 2 > 0.999) in the concentration range of 10-50 µg/ml for ritonavir and 40-200 µg/ml for lopinavir respectively. The slope and intercept for lopinavir was found to be 21201 and 10566 where as for ritonavir was found to be 15278 and 26980 respectively.

Accuracy and precision
Accuracy and precision were determined by elaboration of three standard calibration curves, two from the same day (intra-day) and third one from a different day (inter-day). The intra-day and inter-day precisions (% RSD) at different concentration levels were found to be less than 2% (Table 3). Moreover the % RSD (less variation) showed good precision of the developed HPLC methods.
The respective RP-HPLC area responses from the accuracy determination study are shown in Table 4. Recovery experiment was carried out by applying the standard addition method. Drug assay was performed in triplicate by spiking with equivalent amount of raw material into each volumetric flask for each spike level to get the    concentrations of lopinavir and ritonavir equivalent to 80%, 100%, and 120% of the standard concentrations of lopinavir and ritonavir. The average percentage recovery of both the drugs was found to be within the limits and it is highly accurate.

LOQ and LOD
The LOD and LOQ were determined from the calculated standard deviations of each calibration standard and it was found to be 0.013 µg/ml and 0.465 µg/ml for lopinavir and ritonavir respectively. The calculated LOQ and LOD concentrations confirmed that the method is sensitive.

Specificity
The developed method is specific as none of the excipients interfered with the analytes of interest. Hence, this method is suitably employed for assaying the commercial anti-retroviral individual formulations.

Conclusion
The proposed RP-HPLC is simple, reliable and selective. It also provides satisfactory accuracy and precision with lower limits of detection and quantification. Moreover the shorter duration of analysis for lopinavir and ritonavir make these reported methods suitable for routine quantitative analysis in pharmaceutical dosage forms. The recoveries achieved are good by both the methods.

S.No
Conc of Internal standard (µg/ml)

Area of Internal standard
Conc of ritonavir (µg/ml)