Author(s): Petras T, Siems WG, Grune T
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Abstract The cytotoxic lipid peroxidation product 4-hydroxynonenal (HNE) was infused into rat kidney. During the first 2 min a rapid degradation of a 100 microM HNE solution was demonstrated. After 5 min the consumption rate of 4-HNE reached a steady state of about 75 nmoles/ml. The total HNE consumption rate was about 200 nmoles/g w.w./min. The excretion rate into urine was about 0.1\% of total HNE consumption. It could be demonstrated that the HNE-mercapturic acid formation takes place in the kidney. The formation of the HNE-mercapturic acid contributes up to 6\% to total HNE consumption. Within 10 min of perfusion 2\% of the HNE-mercapturic acid were excreted into urine. The residual 98\% flow back into the blood circulation.
This article was published in Free Radic Biol Med
and referenced in Biochemistry & Pharmacology: Open Access