Author(s): Hartshorne G, Montgomery S, Klentzeris L
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Abstract OBJECTIVE: To study a couple for whom all oocytes failed to mature during three attempts at IVF/ICSI treatment. Our aims were (a) to promote oocyte maturation in vitro and (b) to identify the stage of maturation arrest. DESIGN: Case report. SETTING: Infertility treatment center with academic research links. PATIENT(S): Couple with unexplained infertility. INTERVENTION(S): Exogenous gonadotropin stimulation culminating in the retrieval of eight oocyte-cumulus complexes that were exposed to gonadotropins for 24 hours in vitro. MAIN OUTCOME MEASURE(S): Observation of oocyte cumulus complexes, measurements of oocyte diameter, oocyte chromatin staining with Hoechst 33258. RESULT(S): The cumulus complexes varied in appearance and expanded in vitro, but yielded immature oocytes. Germinal vesicle breakdown occurred in all oocytes and chromatin was consistent with arrest at entry into M-phase. The oocytes were of a size normally competent to mature. CONCLUSION(S): Infertility in this couple was attributed to the failure of oocytes to enter M-phase. This was not overcome by exposure to gonadotropins in vitro nor associated with incompetence due to small oocyte size.
This article was published in Fertil Steril
and referenced in Metabolomics:Open Access