alexa A comparative study of cytomorphology and flow cytometry to detect malignant cells in the body cavity fluid.
Molecular Biology

Molecular Biology

Journal of Cytology & Histology

Author(s): Limvorapitak W, Promsuwicha O, Uauewarakul C

Abstract Share this page

Abstract BACKGROUND: Many types of cancer metastasize to the epithelial linings of the body cavity causing malignant fluid to accumulate in such spaces. Cytomorphological evaluation is considered essential in the diagnosis of malignant body fluid. Nevertheless, the accuracy of cytomorphological results is subjective and can vary depending on the cytopathologists' experience. OBJECTIVE: To determine if DNA content analysis using propidium iodide (PI), anti-CD45 (leukocyte common antigen) and anti-AE1/AE3 (pan-cytokeratin) as analyzed by flow cytometry could be used to detect and differentiate malignant cells in the body fluid when compared to cytomorphological evaluation. MATERIAL AND METHOD: A cross-sectional, laboratory-based, observational study on 90 specimens was conducted. Flow cytometric analysis was done. Sensitivity, specificity, positive predictive value, negative predictive value and accuracy were reported. RESULTS: The DNA index (DI) cut-off value, as determined by the receiver operating characteristic curve of 1.215 or more, had 51.7\% sensitivity and 89.1\% specificity to detect malignant cells. When DI was combined with AE1/AE3 positivity, the sensitivity increased to 62.1\% with 80.3\% specificity. When such techniques were used in adjunct to cytospin preparation, the sensitivity and specificity increased to 89.7\% and 65.66\%, respectively. Twelve specimens (13.3\%) had positive results by flow cytometry but negative cytomorphological results by pathologists, 4 of which were later confirmed as cancer from pleural biopsy. Eleven specimens (12.2\%) had false negativity, 6 of which were unspecified metastatic carcinoma. Four specimens with negative flow cytometric results were cerebrospinal fluid (CSF) specimens with a low cell count. Subgroup analysis in the cases of non-CSF fluid showed 72\% sensitivity and 72.1\% specificity. CONCLUSION: Immunophenotypic analysis using DI and AE1/AE3 in conjunction with cytospin preparation had a moderately high sensitivity to detect malignant cells in the body fluid (-90\%). Non-CSF specimens yielded better results than CSF Further modifications are ongoing in order to increase the detection capabilities of our screening panel.
This article was published in J Med Assoc Thai and referenced in Journal of Cytology & Histology

Relevant Expert PPTs

Relevant Speaker PPTs

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version