alexa A delta2-opioid agonist inhibits p38 MAPK and suppresses activation of murine macrophages.


Virology & Mycology

Author(s): Husted TL, Govindaswami M, Oeltgen PR, Rudich SM, Lentsch AB

Abstract Share this page

Abstract BACKGROUND: delta-Opioid agonists have been shown to attenuate ischemic organ injury in multiple models. The purpose of the present study was to determine if delta-opioid agonists could inhibit proinflammatory cytokine production by macrophages. MATERIAL AND METHODS: Murine macrophages (RAW 264.7) were pretreated for 4 h with media, a dose range (10(-4) to 10(-7) M) of DADLE ([D-Ala2], D-Leu5]-enkephalin, a nonspecific delta-opioid receptor agonist), a dose range (10(-4) to 10(-7) M) of DPDPE ([D2,5Pen]-enkephalin, a specific delta1-opioid receptor agonist), or a dose range (10(-4) to 10(-7) M) of Deltorphin-Dvariant (a specific delta2 opioid receptor agonist) and then incubated with 0.1 microg/ml lipopolysaccharide (LPS) for 1 or 4 h. Cytokine levels were measured by enzyme-linked immunosorbent assay. Activation of NF-kappaB, AP-1, and p38 MAPK were determined by mobility shift assays and Western blot. RESULTS: LPS induced significant increases in TNFalpha and MIP-2 production. Deltorphin-Dvariant, but not DADLE or DPDPE, dose-dependently reduced both TNFalpha and MIP-2 production. Deltorphin-Dvariant did not alter activation of the transcription factors NF-kappaB or AP-1, but greatly reduced activation of p38 MAPK. CONCLUSIONS: The data show that delta2- but not delta1-opioid agonists suppress LPS-induced p38 MAPK activation and expression of TNFalpha and MIP-2. This article was published in J Surg Res and referenced in Virology & Mycology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version