alexa A five year surveillance report on PFGE types of Listeria monocytogenes isolated in Italy from food and food related environments.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Nucera D, Lomonaco S, Bianchi DM, Decastelli L, Grassi MA,

Abstract Share this page

Abstract Listeria monocytogenes can cause severe invasive disease in humans and has been isolated from a variety of foods. This study aimed to investigate type diversity and distribution across sources by subtyping via PFGE a set of 300 L. monocytogenes isolates collected in Italy from foods over a five year period (from 2003 to 2007). The most frequent serotypes were 1/2a (45\%), 1/2c (22\%), and 4b/4e (16\%); 5\% of the isolates were untypeable by conventional serotyping. Significant associations were observed between serotype 1/2a with dairy (O.R.=13.9) and 1/2c with meat (O.R.=33.3). All isolates were typeable, generating 164 combined PFGE profiles. Of these, 121 were unique, being displayed by only one isolate. The other 43 profiles grouped the remaining isolates and were shared between two (N=22), three (N=10), four (N=3) and five isolates (N=4). The remaining 4 profiles were shared between 7, 14, 17 and 46 isolates, respectively. Some profiles (N=7) were retrieved in samples collected in different years, indicating persistency in foods and processing plants. This research may pose the ground for designing a broad typing database which could ease the understanding of L. monocytogenes diversity and could be used for facilitating epidemiological investigations for the identification of listeriosis outbreaks. Data show how large subtype databases may facilitate the identification of common and source-specific types. More comprehensive databases may be needed to fully understand L. monocytogenes diversity and to provide useful data to be considered in epidemiological investigations. Copyright 2010 Elsevier B.V. All rights reserved. This article was published in Int J Food Microbiol and referenced in Journal of Microbial & Biochemical Technology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords