Author(s): Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA,
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Abstract We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70\% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48\% from a single collection and 60\% for all collections combined.
This article was published in Genome Biol
and referenced in Journal of Pharmacogenomics & Pharmacoproteomics