alexa A liquid chromatography tandem mass spectrometric approach for the determination of gangliosides GD3 and GM3 in bovine milk and infant formulae.

Author(s): Srensen LK

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Abstract A liquid chromatographic/tandem mass spectrometric method using pneumatically assisted electrospray ionisation (LC/ESI-MS/MS) was developed for the determination of gangliosides GD3 and GM3 in milk and infant formulae. The gangliosides were extracted in a chloroform/methanol/water environment and cleaned up by solid-phase extraction (SPE) on an end-capped C8 sorbent. The gangliosides were detected in negative ion mode after separation on a reversed-phase (RP) C5 analytical column. From the different ganglioside molecular species, product ions at m/z 290 corresponding to an N-acetylneuraminic acid fragment were produced in the collision cell and used in selected reaction monitoring. A standard addition technique was applied for quantification. The relative repeatability standard deviations were less than 5\% for GD3 (level 10 mg/L) and 14\% for GM3 (level 0.1-0.2 mg/L). Copyright 2006 John Wiley & Sons, Ltd. This article was published in Rapid Commun Mass Spectrom and referenced in

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