alexa A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Proteomics & Bioinformatics

Author(s): Wessel D, Flgge UI

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Abstract A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed. The effectiveness of this method is not affected by the presence of detergents, lipids, salt, buffers, and beta-mercaptoethanol.
This article was published in Anal Biochem and referenced in Journal of Proteomics & Bioinformatics

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