Author(s): Belgrader P, Okuzumi M, Pourahmadi F, Borkholder DA, Northrup MA
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Abstract A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores, and dispenses the mixture into a PCR tube. The total time to automatically process the spores in the cartridge and then detect the spore DNA by real-time PCR was 20 min.
This article was published in Biosens Bioelectron
and referenced in Journal of Bioterrorism & Biodefense