alexa A new approach for in vitro imaging of breast cancer cells by anti-metadherin targeted PVA-pyrene.
Chemical Engineering

Chemical Engineering

Journal of Advanced Chemical Engineering

Author(s): Medine EI, Odaci D, Gacal BN, Gacal B, Sakarya S,

Abstract Share this page

Abstract Poly(vinyl alcohol)-pyrene-anti-metadherin (PVA-Py-(Anti-MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA-Py prepared by "Click chemistry" approach is targeted by Anti-MTDH antibody and applied to the MCF-7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non-targeted structure (PVA-Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous cells. This article was published in Macromol Biosci and referenced in Journal of Advanced Chemical Engineering

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 6th World Congress on Biopolymers
    September 7-9, 2017 Paris, France
  • 2nd Euro Global Summit and Expo on Biomass
    September 21-22, 2017 Madrid, Spain
  • 7th International Congress on Biofuels and Bioenergy
    October 2-4, 2017 Toronto, Canada
  • 3rd International Conference on Chemical Engineering
    October 2-4, 2017 Chicago, USA
  • 7th International Conference and Exhibition on Biopolymers and Bioplastics
    October 19-21, 2017 San Francisco, USA
  • 6th International Congress on Biofuels, Bioenergy and Bioeconomy
    December 04-06, 2017 Sao Paulo, Brazil
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords