alexa A new spectrophotometric method for quantitative multicomponent analysis resolution of mixtures of salicylic and salicyluric acids.
Pharmaceutical Sciences

Pharmaceutical Sciences

Pharmaceutica Analytica Acta

Author(s): Salinas F, Nevado JJ, Mansilla AE

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Abstract A new spectrophotometric method for resolving binary mixtures is proposed. The method is based on use of the first derivative of the ratios of spectra. The absorption spectrum of the mixture is obtained and the amplitudes at appropriate wavelengths are divided by the corresponding amplitudes in the absorption spectrum of a standard solution of one of the components, and the first derivative of the ratio spectrum is obtained. The concentration of the other component is then determined from a calibration graph. The method has been applied for resolving binary mixtures of salicylic and salicyluric acids. Calibration graphs for 2.6-52 ppm salicylic acid and for 2.1-42 ppm salicyluric acid were established by measuring the analytical signals at the maximum at 241.5 nm (for salicylic acid) and from the peak at 258 nm to the trough at 247 nm (for salicyluric acid) in the first derivative ratio spectra.
This article was published in Talanta and referenced in Pharmaceutica Analytica Acta

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