alexa A new technique for orcein banding with acid treatment.
Molecular Biology

Molecular Biology

Journal of Cytology & Histology

Author(s): Chattopadhyay D, Sharma AK

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Abstract A rapid method for banding plant chromosomes using hydrochloric acid with orcein is suggested. The technique has been successful in both dioecious and monoecious families with short chromosomes. It involves pretreatment with an oxyquinoline-paradichlorobenzene mixture, fixation in acetic ethanol and treatment with hydrochloric acid at 60 C followed by staining with orcein. Stained chromosome bands and interbands are reproducible and species specific.
This article was published in Stain Technol and referenced in Journal of Cytology & Histology

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