Author(s): Jia F, Wilson KD, Sun N, Gupta DM, Huang M,
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Abstract Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.
This article was published in Nat Methods
and referenced in Journal of Stem Cell Research & Therapy