alexa A novel enzymatic glucose biosensor and sensitive non-enzymatic hydrogen peroxide sensor based on graphene and cobalt oxide nanoparticles composite modified glassy carbon electrode


Toxicology: Open Access

Author(s): Chelladurai Karuppiah, Selvakumar Palanisamy, ShenMing Chen, Vediyappan Veeramani, Prakash Periakaruppan

Abstract Share this page

In the present study, we have demonstrated the fabrication of novel enzymatic glucose biosensor using glucose oxidase (GOD) as a model enzyme which has been immobilized onto the graphene (GF) and cobalt oxide nanoparticles (Co3O4-NPs) composite modified electrode. The GF/Co3O4-NPs composite was prepared by hydrothermal method and characterized by using scanning electron microscopy, X-ray diffraction and elemental analysis. The GOD immobilized GF/Co3O4-NPs modified electrode shows a well defined redox behaviour indicating the reversible proton and electron transfer reaction of GOD. A heterogeneous electron transfer rate constant (Ks) of immobilized GOD has been calculated to be 3.52 s−1 which is much higher than that of GOD immobilized GF supports. The fast electron transfer of GOD is attributed to the excellent biocompatibility of Co3O4-NPs and high conductivity of the GF. The fabricated glucose biosensor exhibits a wider linear response for glucose from 0.5 mM to 16.5 mM with the sensitivity of 13.52 μA mM−1 cm−2. In addition, a non-enzymatic H2O2 sensor has been further developed using GF/Co3O4-NPs composite modified electrode. The GF/Co3O4-NPs composite electrode shows an excellent electrocatalytic activity towards H2O2 with the response time of <10 s. The H2O2 response at GF/Co3O4-NPs composite modified electrode displays a linear response ranging from 0.2 to 211.5 μM with a limit of detection of 0.06 μM.

This article was published in Sensors and Actuators B: Chemical and referenced in Toxicology: Open Access

Relevant Expert PPTs

Relevant Speaker PPTs

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version