alexa A novel ES cell line, TT2, with high germline-differentiating potency.
Biochemistry

Biochemistry

Journal of Glycobiology

Author(s): Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M,

Abstract Share this page

Abstract In producing mutant mice by gene-targeting and gene-trapping in embryonic stem (ES) cells, the efficient colonization of the mutant ES cells into germline is still a critical matter. We have established a new line of ES cells, TT2, from an F1 embryo between a C57BL/6 female and a CBA male. When the TT2 cells were injected into blastocysts, the colonization into each tissue was very low. However, when injected into eight-cell embryos, the cells segregated inside the blastomeres, localized in an inner cell mass of blastocysts developed 1 day later, and colonized efficiently in each tissue of the pups. The pups were disproportionately male, about half of which were composed of TT2-derived cells primarily; in more than 70\% of the males, TT2-derived cells were dominant, accounting for over half of the total cells. When these males were mated, they exclusively yielded TT2-derived offspring. The germline-differentiating potency was stable during 3 weeks of culture. Twenty-one of 24 mutant clones independently isolated yielded germline chimeras, and 19 clones yielded them in a rate comparable to that of the parent cells. Thus, TT2 cells can serve as a valuable vehicle for the production of mutant mice. This article was published in Anal Biochem and referenced in Journal of Glycobiology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • International Conference on Glycobiology
    Sep 21-22, 2017, Crowne Plaza Houston River Oaks, Houston, USA
  • 2nd International Conference on Biochemistry
    Sep 21-22, 2017 Macau, Hong Kong
  • International Conference on Glycobiology
    Oct 02-04, 2017 Atlanta, USA
  • 4th International Conference on Glycobiology and Glycochemistry
    July 16-18, 2018 Melbourne, Australia
  • 4th Glycobiology World Congress
    September 17-19, 2018 Rome, Italy
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords