alexa A novel rapid method for simultaneous determination of eight active compounds in silymarin using a reversed-phase UPLC-UV detector.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Liu H, Du Z, Yuan Q

Abstract Share this page

Abstract A novel rapid chromatographic method based on utilization of UPLC column was developed for the analysis of eight active compounds in silymarin. The analysis was performed on a Waters Acquity UPLC system with an Acquity UPLC(BEH) C18 column (5mmx2.1mm I.D., 1.7mum) and a gradient elution of methanol and water containing 0.01\% formic acid with a run time of 9min, in which the retention time of the last analyte was 5.8min. And all eight active compounds achieved complete separation. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The results indicated that the type of column, the type of mobile phase and the modified addition were significant to the separation of isomeric compounds in herb extracts. This article was published in J Chromatogr B Analyt Technol Biomed Life Sci and referenced in Journal of Chromatography & Separation Techniques

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords