Author(s): Singh S, Saikia JP, Buragohain AK
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Abstract Alpha-amylase was immobilized onto polyaniline-polyvinyl alcohol (PAni-PVA) film by cross linking with glutaraldehyde. The activity yield and immobilization efficiency was calculated as 13.58\% and 30.60\% respectively. The PAni-PVA-Amylase films were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and amylase activity assay. The immobilized α-amylase had its optimum activity at pH 7.0 and 25°C. The immobilization of α-amylase onto the PAni-PVA films protected the enzyme from Fe(2+) inhibition. The PAni-PVA-Amylase films could be used effectively for 30 cycles with 60\% retention of the activity. Copyright © 2013 Elsevier B.V. All rights reserved.
This article was published in Colloids Surf B Biointerfaces
and referenced in Journal of Microbial & Biochemical Technology