alexa A peroxidase-coupled method for the colorimetric determination of serum triglycerides.
Toxicology

Toxicology

Journal of Environmental & Analytical Toxicology

Author(s): McGowan MW, Artiss JD, Strandbergh DR, Zak B, McGowan MW, Artiss JD, Strandbergh DR, Zak B

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Abstract We describe an enzymatic method for rapid, precise measurement of serum triglycerides with use of sample:reagent ratios as large as 1:200. Hydrolysis of triglycerides is catalyzed by lipase to produce glycerol and free fatty acids. The glycerol generated is then phosphorylated by adenosine 5'-triphosphate in the presence of glycerol kinase. Oxidation of the resulting glycerol 3-phosphate to produce hydrogen peroxide is catalyzed by L-alpha-glycerophosphate oxidase. An intense red chromogen is produced by the peroxidase-catalyzed coupling of 4-aminoantipyrene and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate with hydrogen peroxide. This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.
This article was published in Clin Chem and referenced in Journal of Environmental & Analytical Toxicology

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