alexa A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development.


Journal of Immunobiology

Author(s): Macklon NS, Pieters MH, Hassan MA, Jeucken PH, Eijkemans MJ, , Macklon NS, Pieters MH, Hassan MA, Jeucken PH, Eijkemans MJ, , Macklon NS, Pieters MH, Hassan MA, Jeucken PH, Eijkemans MJ, , Macklon NS, Pieters MH, Hassan MA, Jeucken PH, Eijkemans MJ,

Abstract Share this page

Abstract BACKGROUND: Extending the period of in-vitro culture to the blastocyst stage may improve implantation rates in IVF treatment. Recognition of the dynamic nature of early embryo metabolism has led to the development of commercially available sequential culture systems. However, their improved efficacy over monoculture systems remains to be demonstrated in prospective studies. METHODS: Embryos obtained from 158 women undergoing IVF treatment were randomized by sealed envelopes to culture in one of three systems: (A) culture for 5 days in our own monoculture medium (Rotterdam medium); (B) culture for 3 days in Rotterdam medium followed by 2 days in fresh Rotterdam medium; (C) culture for 5 days using the commercially available G1/G2 sequential culture system. RESULTS: There were no significant differences in blastulation, implantation or pregnancy rates between the three tested culture systems. CONCLUSION: The employed monoculture system is as effective as the G1/G2 sequential system for the culture of blastocysts for IVF.
This article was published in Hum Reprod and referenced in Journal of Immunobiology

Relevant Expert PPTs

Relevant Speaker PPTs

  • Hedef Dhafir El-Yassin
    The Immune Response of Prolactin and the Induction of Tumor Necrosis Factor (TNF) in Iraqi Patients Infected with Hepatitis C Virus
    PPT Version | PDF Version
  • Ahmed Mohammed Ashshi
    The correlations of activins and follistatin with viral load, liver damage, Interleukin-6 and tumour necrosis factor-α in treatment naïve patients with chronic hepatitis C genotype 1 and 4: A case-control study
    PPT Version | PDF Version

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version