alexa A public domain image-analysis program for the single-cell gel-electrophoresis (comet) assay.


Journal of Clinical Toxicology

Author(s): Helma C, Uhl M

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Abstract The single-cell gel electrophoresis (or comet) assay has gained widespread acceptance as a cheap and simple genotoxicity test, but it requires a computer-assisted image-analysis system. As commercial programs are expensive and inflexible, we decided to develop an image-analysis system based on public domain programs and make it publicly available for the scientific community. Our system is based on the scientific image-processing program NIH Image, and was written in its Pascal-like macro language. User interaction was kept as simple as possible, to enable the measurement of a large number of cells with a few keystrokes. Therefore, the time for image analysis is very low, even on slow computers. The comet macro can be obtained from +/comet/, NIH Image is available at Both programs are free of charge.
This article was published in Mutat Res and referenced in Journal of Clinical Toxicology

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